![]() Original Bio-Rad Prestained Protein Standards for SDS-PAGE are available in broad, low, and high ranges. Prestained Natural SDS-PAGE Protein Standards dynamic range in the common loading range for western blot analysis (10-50 g protein of a. Lot-specific MWs are included with each vial. (1610374) and TidyBlot Western Blot Detection Reagent-HRP (STAR209) were purchased from BioRad (Marne-la-Coquette (14). When detecting and quantifying low abundant proteins Bio-Rad recommends customers develop the blots using Bio-Rads Clarity Western ECL Substrate which has long. For IÎ✫α protein detection, the blotting membrane was blocked with 5 BSA (Roth 8076.2) in a tris. * MWs are of representative lots actual weights may vary. A protein ladder was purchased from (Bio-Rad 1610374). Precision Plus Protein WesternC Standards are combination protein standards containing ten prestained, Strep-tagged recombinant proteins, enabling both fluorescent visualization on gels and colorimetric or chemiluminescent western blot detection with StrepTactin-AP or StrepTactin-HRP Conjugate. Kaleidoscope Prestained Natural Standards are broad range and have individually colored proteins to allow instant orientation on SDS-PAGE gels and western blots.Ĭalibrated Molecular Weights* of Kaleidoscope Protein Standards Protein Each lot of Kaleidoscope and SDS-PAGE prestained natural protein standards is individually calibrated for estimating the MW of sample proteins. 3 l or 5 l per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. Prestained Nautral Standards for SDS-PAGE and western blotting provide a quick and easy way to monitor protein separation during electrophoresis and to assess transfer efficiency on blots. ![]()
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